RT Journal
A1 Gaasterland, Terry
A1 Sczyrba, Alexander
A1 Thomas, Elizabeth
A1 Aytekin-Kurban, Gulriz
A1 Gordon, Paul
A1 Sensen, Christoph W.
T1 
MAGPIE/EGRET Annotation of the 2.9-Mb Drosophila melanogaster Adh Region
JF Genome Research 
JO Genome Research 
YR 2000 
FD April 01 
VO 10 
IS 4 
SP 502 
OP 510 
DO 10.1101/gr.10.4.502 
UL http://genome.cshlp.org/content/10/4/502.abstract 
AB Our challenge in annotating the 2.91-Mb Adh region of theDrosophila melanogaster genome was to identify genetic and genomic features automatically, completely, and precisely within a 6-week period. To do so, we augmented the MAGPIE microbial genome annotation system to handle eukaryotic genomic sequence data. The new configuration required the integration of eukaryotic gene-finding tools and DNA repeat tools into the automatic data collection module. It also required us to define in MAGPIEnew strategies to combine data about eukaryotic exon predictions with functional data to refine the exon predictions. At the heart of the resulting new eukaryotic genome annotation system is a reverse comparison of public protein and complementary DNA sequences against the input genome to identify missing exons and to refine exon boundaries. The software modules that add eukaryotic genome annotation capability to MAGPIE are available as EGRET(Eukaryotic Genome RapidEvaluation Tool).