RT Journal
A1 Tripodis, Nicos
A1 Palmer, Sophie
A1 Phillips, Sam
A1 Milne, Sarah
A1 Beck, Stephan
A1 Ragoussis, Jiannis
T1 Construction of a High-Resolution 2.5-Mb Transcript Map of the Human 6p21.2–6p21.3 Region Immediately Centromeric of the Major Histocompatibility Complex
JF Genome Research 
JO Genome Research 
YR 2000 
FD April 01 
VO 10 
IS 4 
SP 454 
OP 472 
DO 10.1101/gr.10.4.454 
UL http://genome.cshlp.org/content/10/4/454.abstract 
AB We have constructed a 2.5-Mb physical and transcription map that spans the human 6p21.2–6p21.3 region and includes the centromeric end of the MHC, using a combination of techniques. In total 88 transcription units including exons, cDNAs, and cDNA contigs were characterized and 60 were confidently positioned on the physical map. These include a number of genes encoding nuclear and splicing factors (Ndr kinase, HSU09564, HSRP20); cell cycle, DNA packaging, and apoptosis related [p21, HMGI(Y), BAK]; immune response (CSBP, SAPK4); transcription activators and zinc finger-containing genes (TEF-5, ZNF76); embryogenesis related (Csa-19); cell signaling (DIPP); structural (HSET), and other genes (TULP1, HSPRARD, DEF-6, EO6811,cyclophilin), as well as a number of RP genes and pseudogenes (RPS10, RPS12-like, RPL12-like, RPL35-like). Furthermore, several novel genes (aBr140-like, a G2S-like, a FBN2-like, aZNF-like, and B1/KIAA0229) have been identified, as well as cDNAs and cDNA contigs. The detailed map of the gene content of this chromosomal segment provides a number of candidate genes, which may be involved in several biological processes that have been associated with this region, such as spermatogenesis, development, embryogenesis, and neoplasia. The data provide useful tools for synteny studies between mice and humans, for genome structure analysis, gene density comparisons, and studies of nucleotide composition, of different isochores and Giemsa light and Giemsa dark bands.