RT Journal
A1 Abdrakhmanov, Igor
A1 Lodygin, Dmitry
A1 Geroth, Paul
A1 Arakawa, Hiroshi
A1 Law, Andy
A1 Plachy, Jiri
A1 Korn, Berndt
A1 Buerstedde, Jean-Marie
T1 A Large Database of Chicken Bursal ESTs as a Resource for the Analysis of Vertebrate Gene Function
JF Genome Research 
JO Genome Research 
YR 2000 
FD December 01 
VO 10 
IS 12 
SP 2062 
OP 2069 
DO 10.1101/gr.1137900 
UL http://genome.cshlp.org/content/10/12/2062.abstract 
AB Chicken B cells create their immunoglobulin repertoire within the Bursa of Fabricius by gene conversion. The high homologous recombination activity is shared by the bursal B-cell-derived DT40 cell line, which integrates transfected DNA constructs at high rates into its endogenous loci. Targeted integration in DT40 is used frequently to analyze the function of genes by gene disruption. In this paper, we describe a large database of >7000 expressed sequence tags (ESTs) from bursal lymphocytes that should be a valuable resource for the identification of gene disruption targets in DT40. ESTs of interest can be recognized easily by online BLAST or keyword searches. Because the database reflects the gene expression profile of bursal lymphocytes, it provides valuable hints as to which genes might be involved in B-cell–specific processes related to immunoglobulin repertoire formation, signal transduction, transcription, and apoptosis. This large collection of chicken ESTs will also be useful for gene expression studies and comparative gene mapping within the chicken genome project. Details of the bursal EST sequencing project and access to database search forms can be found on the DT40 web site (http://genetics.hpi.uni-hamburg.de/dt40.html).[The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AJ392050–AJ399459.]