RT Journal
A1 Kawamoto, Shoko
A1 Yoshii, Junji
A1 Mizuno, Katsuya
A1 Ito, Kouichi
A1 Miyamoto, Yasuhide
A1 Ohnishi, Tadashi
A1 Matoba, Ryo
A1 Hori, Naohiro
A1 Matsumoto, Yuhiko
A1 Okumura, Toshiyuki
A1 Nakao, Yuko
A1 Yoshii, Hisae
A1 Arimoto, Junko
A1 Ohashi, Hiroko
A1 Nakanishi, Hiroko
A1 Ohno, Ikko
A1 Hashimoto, Jun
A1 Shimizu, Kota
A1 Maeda, Kazuhisa
A1 Kuriyama, Hiroshi
A1 Nishida, Koji
A1 Shimizu-Matsumoto, Akiyo
A1 Adachi, Wakako
A1 Ito, Reiko
A1 Kawasaki, Satoshi
A1 Chae, K.S.
A1 Murakawa, Katsuji
A1 Yokoyama, Masahiro
A1 Fukushima, Atsushi
A1 Hishiki, Teruyoshi
A1 Nakaya, Akihiko
A1 Sese, Jun
A1 Monma, Norikazu
A1 Nikaido, Hitoshi
A1 Morishita, Shinichi
A1 Matsubara, Kenichi
A1 Okubo, Kousaku
T1 BodyMap: A Collection of 3′ ESTs for Analysis of Human Gene Expression Information
JF Genome Research 
JO Genome Research 
YR 2000 
FD November 01 
VO 10 
IS 11 
SP 1817 
OP 1827 
DO 10.1101/gr.151500 
UL http://genome.cshlp.org/content/10/11/1817.abstract 
AB BodyMap is a collection of site-directed 3′ expressed sequence tags (ESTs) (gene signatures, GSs) that contains the transcript compositions of various human tissues and was the first systematic effort to acquire gene expression data. For the construction of BodyMap, cDNA libraries were made, preserving abundance information and histologic resolutions of tissue mRNAs. By sequencing 164,000 randomly selected clones, 88,587 GSs that represent chromosomally coded transcripts have been collected from 51 human organs and tissues. They were clustered into 18,722 independent 3′ termini from transcripts, and more than 3000 of these were not found among ESTs assembled in UniGene (Build 75). Assessment of the prevalence of polyadenylation signals and comparison with GenBank cDNAs indicated that there was no significant contamination by internally primed cDNAs or genomic fragments but that there was a relatively high incidence (12%) of alternative polyadenylation sites. We evaluated the sensitivity and resolution of expression information in BodyMap by in silico Northern hybridization and selection of tissue-specific gene probes. BodyMap is a unique resource for estimation of the absolute abundance of transcripts and selection of gene probes for efficient  hybridization-based gene expression profiling. [BodyMap data are available at http://bodymap.ims.u-tokyo.ac.jp.]