TY  - JOUR
A1  - Kawamoto, Shoko
A1  - Yoshii, Junji
A1  - Mizuno, Katsuya
A1  - Ito, Kouichi
A1  - Miyamoto, Yasuhide
A1  - Ohnishi, Tadashi
A1  - Matoba, Ryo
A1  - Hori, Naohiro
A1  - Matsumoto, Yuhiko
A1  - Okumura, Toshiyuki
A1  - Nakao, Yuko
A1  - Yoshii, Hisae
A1  - Arimoto, Junko
A1  - Ohashi, Hiroko
A1  - Nakanishi, Hiroko
A1  - Ohno, Ikko
A1  - Hashimoto, Jun
A1  - Shimizu, Kota
A1  - Maeda, Kazuhisa
A1  - Kuriyama, Hiroshi
A1  - Nishida, Koji
A1  - Shimizu-Matsumoto, Akiyo
A1  - Adachi, Wakako
A1  - Ito, Reiko
A1  - Kawasaki, Satoshi
A1  - Chae, K.S.
A1  - Murakawa, Katsuji
A1  - Yokoyama, Masahiro
A1  - Fukushima, Atsushi
A1  - Hishiki, Teruyoshi
A1  - Nakaya, Akihiko
A1  - Sese, Jun
A1  - Monma, Norikazu
A1  - Nikaido, Hitoshi
A1  - Morishita, Shinichi
A1  - Matsubara, Kenichi
A1  - Okubo, Kousaku
T1  - BodyMap: A Collection of 3′ ESTs for Analysis of Human Gene Expression Information
Y1  - 2000/11/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1817 
EP  - 1827 
DO  - 10.1101/gr.151500 
VL  - 10 
IS  - 11 
UR  - http://genome.cshlp.org/content/10/11/1817.abstract 
N2  - BodyMap is a collection of site-directed 3′ expressed sequence tags (ESTs) (gene signatures, GSs) that contains the transcript compositions of various human tissues and was the first systematic effort to acquire gene expression data. For the construction of BodyMap, cDNA libraries were made, preserving abundance information and histologic resolutions of tissue mRNAs. By sequencing 164,000 randomly selected clones, 88,587 GSs that represent chromosomally coded transcripts have been collected from 51 human organs and tissues. They were clustered into 18,722 independent 3′ termini from transcripts, and more than 3000 of these were not found among ESTs assembled in UniGene (Build 75). Assessment of the prevalence of polyadenylation signals and comparison with GenBank cDNAs indicated that there was no significant contamination by internally primed cDNAs or genomic fragments but that there was a relatively high incidence (12%) of alternative polyadenylation sites. We evaluated the sensitivity and resolution of expression information in BodyMap by in silico Northern hybridization and selection of tissue-specific gene probes. BodyMap is a unique resource for estimation of the absolute abundance of transcripts and selection of gene probes for efficient  hybridization-based gene expression profiling. [BodyMap data are available at http://bodymap.ims.u-tokyo.ac.jp.] 
ER  -