RT Journal
A1 Huminiecki, Lukasz
A1 Bicknell, Roy
T1 In Silico Cloning of Novel Endothelial-Specific Genes
JF Genome Research 
JO Genome Research 
YR 2000 
FD November 01 
VO 10 
IS 11 
SP 1796 
OP 1806 
DO 10.1101/gr.150700 
UL http://genome.cshlp.org/content/10/11/1796.abstract 
AB The endothelium plays a pivotal role in many physiological and pathological processes and is known to be an exceptionally active transcriptional site. To advance our understanding of endothelial cell biology and to elucidate potential pharmaceutical targets, we developed a new database screening approach to permit identification of novel endothelial-specific genes. The UniGene gene index was screened using high stringency BLAST against a pool of endothelial expressed sequence tags (ESTs) and a pool of nonendothelial ESTs constructed from cell-type–specific dbEST libraries. UniGene clusters with matches in the endothelial pool and no matches in the nonendothelial pool were selected. The UniGene/EST approach was then combined with serial analysis of gene expression (SAGE) library subtraction and reverse transcription polymerase chain reaction to further examine interesting clusters. Four novel genes were identified and labeled: endothelial cell–specific molecules (ECSM) 1–3 and magic roundabout (similar to the axon guidance protein roundabout). In summary, we present a powerful novel approach for comparative expression analysis combining two datamining strategies followed by experimental verification.