RT Journal
A1 Konfortov, Bernard A.
A1 Cohen, Helen M.
A1 Bankier, Alan T.
A1 Dear, Paul H.
T1 A High-Resolution HAPPY Map of Dictyostelium discoideum Chromosome 6
JF Genome Research 
JO Genome Research 
YR 2000 
FD November 01 
VO 10 
IS 11 
SP 1737 
OP 1742 
DO 10.1101/gr.141700 
UL http://genome.cshlp.org/content/10/11/1737.abstract 
AB We have made a high-resolution HAPPY map of chromosome 6 ofDictyostelium discoideum consisting of 300 sequence-tagged sites with an average spacing of 14 kb along the ∼4-Mb chromosome. The majority of the marker sequences were derived from randomly chosen clones from four different chromosome 6–enriched plasmid libraries or from subclones of YACs previously mapped to chromosome 6. The map appears to span the entire chromosome, although marker density is greater in some regions than in others and is lowest within the telomeric region. Our map largely supports previous gene-based maps of this chromosome but reveals a number of errors in the physical map. In addition, we find that a high proportion of the plasmid sequences derived from gel-enriched chromosome 6 (and that form the basis of a chromosome-specific sequencing project) originates from other chromosomes.[The sequence data described in this paper have been submitted to the GenBank data library under accession nos.G64996–G65046.]