RT Journal
A1 Carninci, Piero
A1 Shibata, Yuko
A1 Hayatsu, Norihito
A1 Sugahara, Yuichi
A1 Shibata, Kazuhiro
A1 Itoh, Masayoshi
A1 Konno, Hideaki
A1 Okazaki, Yasushi
A1 Muramatsu, Masami
A1 Hayashizaki, Yoshihide
T1 Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes
JF Genome Research 
JO Genome Research 
YR 2000 
FD October 01 
VO 10 
IS 10 
SP 1617 
OP 1630 
DO 10.1101/gr.145100 
UL http://genome.cshlp.org/content/10/10/1617.abstract 
AB In the effort to prepare the mouse full-length cDNA encyclopedia, we previously developed several techniques to prepare and select full-length cDNAs. To increase the number of different cDNAs, we introduce here a strategy to prepare normalized and subtracted cDNA libraries in a single step. The method is based on hybridization of the first-strand, full-length cDNA with several RNA drivers, including starting mRNA as the normalizing driver and run-off transcripts from minilibraries containing highly expressed genes, rearrayed clones, and previously sequenced cDNAs as subtracting drivers. Our method keeps the proportion of full-length cDNAs in the subtracted/normalized library high. Moreover, our method dramatically enhances the discovery of new genes as compared to results obtained by using standard, full-length cDNA libraries. This procedure can be extended to the preparation of full-length cDNA encyclopedias from other organisms.