TY  - JOUR
A1  - Carninci, Piero
A1  - Shibata, Yuko
A1  - Hayatsu, Norihito
A1  - Sugahara, Yuichi
A1  - Shibata, Kazuhiro
A1  - Itoh, Masayoshi
A1  - Konno, Hideaki
A1  - Okazaki, Yasushi
A1  - Muramatsu, Masami
A1  - Hayashizaki, Yoshihide
T1  - Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes
Y1  - 2000/10/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1617 
EP  - 1630 
DO  - 10.1101/gr.145100 
VL  - 10 
IS  - 10 
UR  - http://genome.cshlp.org/content/10/10/1617.abstract 
N2  - In the effort to prepare the mouse full-length cDNA encyclopedia, we previously developed several techniques to prepare and select full-length cDNAs. To increase the number of different cDNAs, we introduce here a strategy to prepare normalized and subtracted cDNA libraries in a single step. The method is based on hybridization of the first-strand, full-length cDNA with several RNA drivers, including starting mRNA as the normalizing driver and run-off transcripts from minilibraries containing highly expressed genes, rearrayed clones, and previously sequenced cDNAs as subtracting drivers. Our method keeps the proportion of full-length cDNAs in the subtracted/normalized library high. Moreover, our method dramatically enhances the discovery of new genes as compared to results obtained by using standard, full-length cDNA libraries. This procedure can be extended to the preparation of full-length cDNA encyclopedias from other organisms. 
ER  -