@article{Carninci01102000,
author = {Carninci, Piero and Shibata, Yuko and Hayatsu, Norihito and Sugahara, Yuichi and Shibata, Kazuhiro and Itoh, Masayoshi and Konno, Hideaki and Okazaki, Yasushi and Muramatsu, Masami and Hayashizaki, Yoshihide}, 
title = {Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes},
volume = {10}, 
number = {10}, 
pages = {1617-1630}, 
year = {2000}, 
doi = {10.1101/gr.145100}, 
abstract ={In the effort to prepare the mouse full-length cDNA encyclopedia, we previously developed several techniques to prepare and select full-length cDNAs. To increase the number of different cDNAs, we introduce here a strategy to prepare normalized and subtracted cDNA libraries in a single step. The method is based on hybridization of the first-strand, full-length cDNA with several RNA drivers, including starting mRNA as the normalizing driver and run-off transcripts from minilibraries containing highly expressed genes, rearrayed clones, and previously sequenced cDNAs as subtracting drivers. Our method keeps the proportion of full-length cDNAs in the subtracted/normalized library high. Moreover, our method dramatically enhances the discovery of new genes as compared to results obtained by using standard, full-length cDNA libraries. This procedure can be extended to the preparation of full-length cDNA encyclopedias from other organisms.}, 
URL = {http://genome.cshlp.org/content/10/10/1617.abstract}, 
eprint = {http://genome.cshlp.org/content/10/10/1617.full.pdf+html}, 
journal = {Genome Research} 
}