RT Journal
A1 Singer-Sam, J
A1 LeBon, J M
A1 Dai, A
A1 Riggs, A D
T1 A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.
JF Genome Research 
JO Genome Research 
YR 1992 
FD February 01 
VO 1 
IS 3 
SP 160 
OP 163 
DO 10.1101/gr.1.3.160 
UL http://genome.cshlp.org/content/1/3/160.abstract 
AB We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.