TY - JOUR A1 - Singer-Sam, J A1 - LeBon, J M A1 - Dai, A A1 - Riggs, A D T1 - A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide. Y1 - 1992/02/01 JF - Genome Research JO - Genome Research SP - 160 EP - 163 DO - 10.1101/gr.1.3.160 VL - 1 IS - 3 UR - http://genome.cshlp.org/content/1/3/160.abstract N2 - We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript. ER -