@article{Singer-Sam01021992, author = {Singer-Sam, J and LeBon, J M and Dai, A and Riggs, A D}, title = {A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.}, volume = {1}, number = {3}, pages = {160-163}, year = {1992}, doi = {10.1101/gr.1.3.160}, abstract ={We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.}, URL = {http://genome.cshlp.org/content/1/3/160.abstract}, eprint = {http://genome.cshlp.org/content/1/3/160.full.pdf+html}, journal = {Genome Research} }