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  2. ...fusions or fissions. Also, the comparison of T. panzhihuanense with the long-read-based high-quality Morchella sextelata M. Kuo assembly revealed relatively high levels of mesosynteny conservation between the two species (Fig. 5B; Supplemental Fig. S14B). Indeed, 61% of the T. panzhihuanense and 70...
  3. ...learning model to reveal the distinct motif configuration of S. pombe poly(A) sites, which show more precise cleavage than S. cerevisiae. Altogether, our deep learning models provide unprecedented insights into poly(A) site formation of yeast species, and our results highlight divergent poly(A) signals...
  4. ...in gene expression and orchestrate production, function, and turnover of mRNAs. The accuracy and dynamics of RNA–protein interactions within these molecular machines are essential for their function and are mediated by RNA-binding proteins (RBPs). Here, we show that fission yeast whole-cell poly(A)+ RNA...
  5. ...elements in this key process of the central dogma of molecular biology.ResultsEvolutionary dynamics of transcription initiation landscapes in yeastsWe generated high-resolution TSS maps for 10 budding yeast species and two fission yeast species, including S. cerevisiae, S. pombe, and other important...
  6. ...using no-amplification nontagging Cap analysis of gene expression (nAnT-iCAGE) sequencing. We mapped ∼1 million well-supported TSSs, suggesting highly pervasive transcription initiation in the compact of the budding yeast. The comprehensive TSS maps allowed us to identify core promoters for ∼96...
  7. ...association patternsIn yeast, in which many RNAPII genes are transcriptionally regulated, a group of chromatin regulators are enriched specifically at promoters, where they assist in transcription initiation, whereas others travel with RNAPII into gene bodies, aiding transcription elongation, splicing...
  8. ...in chromatin during the transcriptional response to heat shock in the budding yeast Saccharomyces cerevisiae revealed nucleosome eviction in promoter regions but only minor effects in coding regions. Here, we describe the short-term response to nitrogen starvation in the fission yeast Schizosaccharomyces...
  9. ...Ribosome profiling reveals post-transcriptional buffering of divergent gene expression in yeast C. Joel McManus 1 , Gemma E. May , Pieter Spealman and Alan Shteyman Carnegie Mellon University, Department of Biological Sciences, Pittsburgh...
  10. ...to the general belief that it does not occur. Although the negative elongation factor (NELF), absent in budding yeast, is linked to stable transcriptional pausing, it is not essential as shown in Schizosaccharomyces pombe (Booth et al. 2018; Core and Adelman 2019). To our knowledge, few reports of promoter...
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