Figure 4.
Detection of a segregating mutation in R12A. The three chromatograms show DNA sequence data for the single instance where we were able to detect evidence for both ancestral DNA and recovery-line mutations. The mutation at position 12,610,210 on chr II (shared with R12C), indicated by the asterisk, was first detected as a segregating variant at generation 40 in R12A then appeared in a fixed state at generation 50. There was no evidence for this mutation at generation 30. We were unable to detect a segregating variant for any of the other 27 recovery-line mutations.
