
Schematic of the MA12 recovery-line system. The seven genotypes considered are shown in circles, and arrows represent generational time. MA12 was bottlenecked as single hermaphrodite nematodes across 323 generations and each of the five MA12-derived recovery lines (R12A–R12E) evolved in the lab for 60 generations at much larger (N > 1000 nematodes) population sizes. All genotypes were analyzed by Illumina sequencing, with the exception of the two recovery lines indicated by asterisks.











