MPERs in Various Genomic Regions
| Gene/location | Characteristics of the region analyzed | MPER (number of errors/site/21.6 generations) | Fidelity (%/site/generation) | ||||
| G + C content (%) | CpG score | Methylation status | |||||
| E-cadherin | |||||||
| CGI-P | 64.6 | 0.70 | U | 0.022 ± 0.012 | 99.89 | ||
| CGI-outside | 66.7 | 0.66 | U | 0.053 ± 0.012*** | 99.75 | ||
| Non-CGI | 50.2 | 0.59 | M | 0.004 ± 0.005* | 99.98 | ||
| p41-Arc | |||||||
| CGI-P | 68.9 | 0.69 | U | 0.032 ± 0.010 | 99.85 | ||
| CGI-outside | 66.4 | 0.72 | U | 0.091 ± 0.039** | 99.56 | ||
| Non-CGI | 65.2 | 0.31 | M | 0.017 ± 0.012* | 99.92 | ||
| SIM2 | |||||||
| CGI-P | 65.7 | 0.76 | U | 0.018 ± 0.004 | 99.92 | ||
| CGI-outside | 64.8 | 0.70 | U | 0.037 ± 0.008*** | 99.83 | ||
| 3-OST-2 | |||||||
| CGI-P | 66.1 | 0.80 | U | 0.021 ± 0.008 | 99.90 | ||
| Cyclophilin A | |||||||
| CGI-P | 61.6 | 0.90 | U | 0.032 ± 0.017 | 99.85 | ||
| MAGE-A3 | |||||||
| CGI-P | 63.7 | 0.46 | M | 0.002 ± 0.004 | 99.99 | ||
| H19-DMR | |||||||
| both alleles | 59.5 | 0.65 | U/M | 0.026 ± 0.016 | 99.87 | ||
| unmethylated | U | 0.043 ± 0.034 | 99.80 | ||||
| methylated | M | 0.007 ± 0.011* | 99.98 | ||||
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MPERs (number of errors/site/21.6 generations) were calculated from the observed number of errors in six clonal populations that underwent 21.6 generations. Fidelity (%/site/generation) was calculated by the equation M = 1 −F 21.6. MPERs in CGIs outside the promoter regions and non-CGI regions were compared with those in the promoter regions by the t-test, *P < 0.05, **P < 0.01, ***P < 0.005. CGI-P: CGI in the promoter region; CGI-outside: CGI outside the promoter regions; Non-CGI: CpG sites outside CGIs. G + C content and CpG score of the regions analyzed were calculated for the most suitable region larger than 500 bp using a program at the “CpG Island Searcher” web site (http://www.uscnorris.com/cpgislands/). Methylation statuses of these regions in physiological conditions were described as U: unmethylated, M: methylated.
