This Article OPEN ACCESS ARTICLE Full Text Full Text (PDF) Supplemental Research Data All Versions of this Article: gr.3739005v1 15/5/641    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Irwin, B. Articles by Sandmeyer, S. Search for Related Content PubMed PubMed Citation Articles by Irwin, B. Articles by Sandmeyer, S. Social Bookmarking                   What's this?

Retroviruses and yeast retrotransposons use overlapping sets of host genes

¹ Department of Biological Chemistry, UC Irvine, Irvine, California 92697, USA ² School of Information and Computer Science, UC Irvine, Irvine, California 92697, USA ³ Institute for Genomics and Bioinformatics, UC Irvine, Irvine, California 92697, USA

A collection of 4457 Saccharomyces cerevisiae mutants deleted for nonessential genes was screened for mutants with increased or decreased mobilization of the gypsylike retroelement Ty3. Of these, 64 exhibited increased and 66 decreased Ty3 transposition compared with the parental strain. Genes identified in this screen were grouped according to function by using GOnet software developed as part of this study. Gene clusters were related to chromatin and transcript elongation, translation and cytoplasmic RNA processing, vesicular trafficking, nuclear transport, and DNA maintenance. Sixty-six of the mutants were tested for Ty3 proteins and cDNA. Ty3 cDNA and transposition were increased in mutants affected in nuclear pore biogenesis and in a subset of mutants lacking proteins that interact physically or genetically with a replication clamp loader. Our results suggest that nuclear entry is linked mechanistically to Ty3 cDNA synthesis but that host replication factors antagonize Ty3 replication. Some of the factors we identified have been previously shown to affect Ty1 transposition and others to affect retroviral budding. Host factors, such as these, shared by distantly related Ty retroelements and retroviruses are novel candidates for antiviral targets.

Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.3739005. Article published online ahead of print in April 2005. Freely available online through the Genome Research Immediate Open Access option.

⁴ These authors contributed equally to this work.

⁵ Corresponding author.
E-mail sbsandme{at}uci.edu; fax (949) 824-2688.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. Servant, C. Pennetier, and P. Lesage Remodeling Yeast Gene Transcription by Activating the Ty1 Long Terminal Repeat Retrotransposon under Severe Adenine Deficiency Mol. Cell. Biol., September 1, 2008; 28(17): 5543 - 5554. [Abstract] [Full Text] [PDF]

				K. M. Nyswaner, M. A. Checkley, M. Yi, R. M. Stephens, and D. J. Garfinkel Chromatin-Associated Genes Protect the Yeast Genome From Ty1 Insertional Mutagenesis Genetics, January 1, 2008; 178(1): 197 - 214. [Abstract] [Full Text] [PDF]

				M. J. Curcio, A. E. Kenny, S. Moore, D. J. Garfinkel, M. Weintraub, E. R. Gamache, and D. T. Scholes S-Phase Checkpoint Pathways Stimulate the Mobility of the Retrovirus-Like Transposon Ty1 Mol. Cell. Biol., December 15, 2007; 27(24): 8874 - 8885. [Abstract] [Full Text] [PDF]

				S. Sistla, J. V. Pang, C. X. Wang, and D. Balasundaram Multiple Conserved Domains of the Nucleoporin Nup124p and Its Orthologs Nup1p and Nup153 Are Critical for Nuclear Import and Activity of the Fission Yeast Tf1 Retrotransposon Mol. Biol. Cell, September 1, 2007; 18(9): 3692 - 3708. [Abstract] [Full Text] [PDF]

				L. S. Z. Larsen, M. Zhang, N. Beliakova-Bethell, V. Bilanchone, A. Lamsa, K. Nagashima, R. Najdi, K. Kosaka, V. Kovacevic, J. Cheng, et al. Ty3 Capsid Mutations Reveal Early and Late Functions of the Amino-Terminal Domain J. Virol., July 1, 2007; 81(13): 6957 - 6972. [Abstract] [Full Text] [PDF]

				P. H. Maxwell and M. J. Curcio Host Factors That Control Long Terminal Repeat Retrotransposons in Saccharomyces cerevisiae: Implications for Regulation of Mammalian Retroviruses Eukaryot. Cell, July 1, 2007; 6(7): 1069 - 1080. [Full Text] [PDF]

				S. Gallois-Montbrun, B. Kramer, C. M. Swanson, H. Byers, S. Lynham, M. Ward, and M. H. Malim Antiviral Protein APOBEC3G Localizes to Ribonucleoprotein Complexes Found in P Bodies and Stress Granules J. Virol., March 1, 2007; 81(5): 2165 - 2178. [Abstract] [Full Text] [PDF]

				D. C. Presgraves and W. Stephan Pervasive Adaptive Evolution among Interactors of the Drosophila Hybrid Inviability Gene, Nup96 Mol. Biol. Evol., January 1, 2007; 24(1): 306 - 314. [Abstract] [Full Text] [PDF]

				S. L. Sawyer and H. S. Malik Eukaryotic Transposable Elements and Genome Evolution Special Feature: Positive selection of yeast nonhomologous end-joining genes and a retrotransposon conflict hypothesis PNAS, November 21, 2006; 103(47): 17614 - 17619. [Abstract] [Full Text] [PDF]

				C. Tagwerker, K. Flick, M. Cui, C. Guerrero, Y. Dou, B. Auer, P. Baldi, L. Huang, and P. Kaiser A Tandem Affinity Tag for Two-step Purification under Fully Denaturing Conditions: Application in Ubiquitin Profiling and Protein Complex Identification Combined with in vivoCross-Linking Mol. Cell. Proteomics, April 1, 2006; 5(4): 737 - 748. [Abstract] [Full Text] [PDF]

				N. BELIAKOVA-BETHELL, C. BECKHAM, T. H. GIDDINGS JR, M. WINEY, R. PARKER, and S. SANDMEYER Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components RNA, January 1, 2006; 12(1): 94 - 101. [Abstract] [Full Text] [PDF]

				E. R. Havecker, X. Gao, and D. F. Voytas The Sireviruses, a Plant-Specific Lineage of the Ty1/copia Retrotransposons, Interact with a Family of Proteins Related to Dynein Light Chain 8 Plant Physiology, October 1, 2005; 139(2): 857 - 868. [Abstract] [Full Text] [PDF]